The structure and the basic directions of CLC5958 are introduced in this paper in detail. Then the design proposal of a high-speed data acquisition card based on FPGA&PCI is proposed, which is proved to be feasible after simulation. This card can acquire the data with high accuracy at a high speed, and has a good anti-interference performance too. The card’s configuration is simple and it’s convenient to enlarge the function. This acquisition card is applicable to intelligent instruments and other occasions that need high-speed data acquisition.