Abstract: Firstly, the hierarchy and work principle of the multifunctional testing system based on USB 2.0 are introduced in this article. Then taking data acquisition module,data storage module , USB 2.0 interface for example,it discusses in detail the function and design of the system hardware. In the end,the system software structure,the design of instrument driver and virtual instrument software are analyzed in detail. Key words: Testing System;Data Acquisition Module;Data Storage Module;USB 2.0